High-speed signals have been a hot topic that can't be avoided by communication industries. With the increase on amount of transmitted information and transmission speed rate, high-speed signals have become gradually significant. High-speed PCB (Printed Circuit Board) is a loading board of high-speed signals and its material selection, manufacturing technology and routing design all affect quality of high-speed signals. Non-Functional Pad, short for NFP, is a technological method to manufacturing high-speed PCB while insertion loss is one of the most important parameters indicating signal quality. To remove or to keep NFP has been an inevitable discussion topic between engineers and manufacturers. In this article, influence of NFP on insertion loss of high-speed signals will be analyzed from the perspective of manufacturing procedure in an experimental method. Whether to remove or to keep unused pads, you can find an answer in this article.